Journal: Cancer Science
Article Title: G protein pathway suppressor 2 suppresses gastric cancer by destabilizing epidermal growth factor receptor
doi: 10.1111/cas.15151
Figure Lengend Snippet: G protein pathway suppressor 2 (GPS2) is expressed at low levels in gastric cancer (GC) and low GC expression is associated with poor prognosis. A, Western blot analysis of the GPS2 protein levels in the GES1 and GC cell lines. B, Determination of the GPS2 mRNA levels in 16 tumor tissues and paired noncancerous normal tissues from GC patients by quantitative PCR. ∆Ct (N), the Ct value of GAPDH was subtracted from the Ct value of GPS2 in normal gastric tissues. ∆Ct (T), the Ct value of GAPDH was subtracted from the Ct value of GPS2 in the cancer tissues. The bar value (∆Ct (N) – ∆Ct (T)) represents the difference in the GPS2 mRNA levels between the normal tissues and paired tumor tissues. Bar value = −1 indicates that the GPS2 mRNA expression of the tumor tissues is 2 −1 ‐fold of that of the paired normal tissues, showing that the expression of GPS2 is decreased in the tumors. Bar value = 1 indicates that the GPS2 mRNA expression of the tumor tissues is 2 1 ‐fold of that of the paired normal tissues, showing that the expression of GPS2 is increased in the tumors. C, Western blot analyses of GPS2 protein expression in 10 tumor tissues (T) and paired noncancerous normal tissues (N) from GC patients (P#1‐P#10). D, Immunohistochemical (IHC) analysis of the GPS2 protein levels in GC tissues and normal tissues on a tissue microarray. GC tissue sections were quantitatively scored according to the percentage of positive cells and staining intensity. E, Representative images of IHC staining of GPS2 protein expression in normal tissues and two different GC tissues. F, IHC analysis of GPS2 protein levels in 30 paired GC tissues. G, Relative proportion of GC patients with low GPS2 expression in T, N, and clinical stages. H, Survival curves of GC patients with low GPS2 expression vs GC patients with high GPS2 expression. * P < .05; *** P < .001
Article Snippet: The GC lines MKN74, SGC7901, BGC823, MGC803, HGC27, and AGS and normal human gastric epithelial cell line GES1 were purchased from ATCC.
Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Microarray, Staining, Immunohistochemistry
